Identification of Four Novel Synonymous Substitutions in the X-Linked Genes Neuroligin 3 and Neuroligin 4X in Japanese Patients with Autistic Spectrum Disorder

Table 2

Primer sets used to screen for variants by PCR-HRM analysis.

Gene	Exon	Forward	Reverse
NLGN3	2.1	GCTCAGTTTTGAGGTTCAAGTC	TCACTGGGCAGTGGTACTCG
	2.2	CACAGTCAACACTCACTTTGG	GATGGTTAGAAGCATTTTCACAG
	3	GGCAGAGGCCTCCTGTTATT	CAAATCCTCCCTGCAAGGCA
	4	TGGCTTGCTGGGCCACACTG	GCCAAAGACAGATGAACAGCC
	5	AGGTTGAGCAACCCCATGAGT	GGGCCAGAGGATAACACCATT
	6	CATCCCTCTGCCTTCATTGTC	TAGAAGAGAGCTGGCCGATTC
	7.1	CAGCCTCAGTGACAAAGGAAT	CAGGGTGTCCTTACCCTCAG
	7.2	GTAAGGACACCCTGCGAGAG	TGGGGTCTCAAAGAGGAAAA
	8.1	GTGACCCCAGATTTCCATGT	GGCCAGAACGTTAAGGAACA
	8.2	ATCACCCGCAGGCCCAATGG	CCTCACACTCGTGGTGGGTG
	8.3	GGAGGAGCTGGCAGCATTAC	CTGGAGATTGGCTGTGCTCT
NLGN4X	2.1	AAAGCCCTATCTCTCTGCAGG	TGAGTAGTATTTCGGATGCCAG
	2.2	AAGAACACCGTTACCCAATGAG	GAGACATTATAAAACCCTCCTAG
	3	TTAGCATTGGTGAGTCAGTGTG	CCGTCAAAACGAGAAGTGGACT
	4	CTTTTTCTATTTGGCCACCA	TTCTTGGTTCAGGGTATTTGC
	5.1	AGCTGCATTTCTGTCCTGTG	TCTCCCGCAAAGTGTCTTTC
	5.2	CCAACTTCGTGGACAACCTT	ACCCCAACACGAAGATGAAC
	6.1	CACGTCACATGTGGAAGAGT	GACGGCAATGGTGACACTTA
	6.2	TCCTCATTGAAACCAAACGA	AACATTCCTGGTCTGGAGAC