Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells
Figure 1
Relevant peptide and DNA sequences. (a) 42-residue HIV-1 enhancer-binding peptide (R42) derived from the DNA-binding domain of 434 repressor. The recognition helix in (a), (b), and (c) is underlined. (b) 64-residue HIV-1 enhancer-binding peptide obtained through extension of R42 by the nuclear localization signal of SV40 large T antigen and nona-arginine (NLS and Arg9 underlined). (c) 62-residue HIV-1 enhancer-binding peptide obtained through extension of R42 at the N-terminus by the bipartite nuclear localization signal of Hairless (underlined) embracing protein transduction domain-5 (PTD-5, italics). (d) 20-residue control peptide containing only NLS of SV40 large T antigen and Arg9 linked by two glycine residues (NLS and Arg9 underlined). (e) 70-bp HIV-1 LTR DNA comprising positions −137 to −68. The two identical NF-κB-binding sequences (enhancers) are underlined. (f) 51-bp competitor DNA lacking the two enhancers, otherwise it is largely identical with the 70-bp HIV-1 LTR DNA shown in (e).
