Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells
Figure 2
Competitive band shift assays of the interactions of R64 with 32P-labeled HIV-1 LTR DNA (Figure 1(e)) in the presence of increasing amounts of unlabeled competitor DNA (Figure 1(f)). Samples (20 μL each) were incubated in 50 mM phosphate buffer, pH 7.4, for 30 min at 37°C [21] and then submitted to polyacrylamide gel electrophoresis at 250 V in 33 mM Tris, 0.75 mM EDTA, adjusted to pH 8.2 using boric acid. R64; lanes 1 to 4 (8 pmol each); competitor DNA; lanes 1 to 4 (1, 4.25, 8.5, 17 pmol, resp.); labeled enhancer-containing DNA (target DNA); lanes 1 to 5 (200 fmol each).
