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Advances in Virology
Volume 2011 (2011), Article ID 272193, 12 pages
http://dx.doi.org/10.1155/2011/272193
Research Article

Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples

1HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USA
2Protein Expression Laboratory, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USA
3Urologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA
4UC Davis Cancer Center, Sacramento, CA 95817, USA
5AIDS and Cancer Virus Program, SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD 21702, USA
6Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USA
7Department of Molecular Biology and Microbiology, Tufts University, Boston, MA 02155, USA

Received 21 June 2011; Revised 8 August 2011; Accepted 27 August 2011

Academic Editor: Yoshinao Kubo

Copyright © 2011 M. F. Kearney et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick), we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replication competent virus. Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. Using these assays, we did not detect evidence of XMRV in blood samples ( ) or prostate specimens ( ) from two independent cohorts of patients with prostate cancer. Previous studies detected XMRV in prostate tissues. In the present study, we primarily investigated the levels of XMRV in blood plasma samples collected from patients with prostate cancer. These results demonstrate that while XMRV-related assays developed at the NCI-Frederick can readily measure XMRV nucleic acids, antibodies, and replication competent virus, no evidence of XMRV was found in the blood of patients with prostate cancer.