Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors

<table>Schematic diagram of EYFP fusion proteins.  The N-terminal half of EYFP (aa 1–173) was joined in-frame to full-length Tsg101, and the C-terminal half of EYFP (aa 174–239) was joined in-frame to the indicated WT or L-domain mutant VP40 or HIV-1 Gag proteins.  A FLAG epitope tag is positioned at the N-terminus of mVP40-WT, and between the CYFP fragment and mVP40 in construct CYFP-mVP40-WT.  The CYFP-Gag-ΔP6 construct was made by fusing the CYFP fragment in-frame with Gag L-domain deletion mutant (the entire p6 region containing L-domain motif PTAP is deleted).</table>

Advances in Virology

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Figure 1

Figure 1: Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors 

Figure 1 | Bimolecular Complementation to Visualize Filovirus VP40-Host Complexes in Live Mammalian Cells: Toward the Identification of Budding Inhibitors 