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Figure 3: Dual color TIRFM of SINV and Rab-5 after endocytosis. A SINV virus after entering the cell was tracked (red). After approximately 90 seconds, it was associated with GFP-Rab5 (green). The association lasted ~60 second before the GFP-Rab5 dissociated and the SINV moved deeper into the cell. Time in seconds is labeled. Arrowheads indicate the association of Rab5. The last image shows an overlay composite picture of the cell.