Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs
Figure 3
IgG and IgA antibody responses in serum and nasal wash samples. Pre- and postvaccination serum samples were collected every other week for six weeks from animals vaccinated i.n. (a) or s.c. (b) and were assayed for immunogen (left to right: ovalbumin, TCA peptide, ntPE-LTA2B-GH, LTA2B-GH) IgG responses by ELISA. Nasal washes were collected at the same time points and animals vaccinated i.n. (c) or s.c. (d) were assayed for immunogen IgA responses by ELISA. X-axis represents time in weeks, that is, from 0 to 6 weeks. Y-axis represents OD @ 450 nm. Statistical analysis was performed using ANOVA and Tukey’s mean comparison test. Significance determined by (*) representing , (**) , and (***) .
