No Evidence of XMRV or MuLV Sequences in Prostate Cancer, Diffuse Large B-Cell Lymphoma, or the UK Blood Donor Population
Table 1
Details of PCRs used to test blood sample.
Sample tested
PCR
Target
Primers and probes
Cycles ()
Reagents
540 DNAs from whole blood
XMRV Taq Man
gag
XMRV Probe, F, R
60
Qiagen QuantiTect Probe kit
540 DNAs from whole blood
SBCMV Taq Man
SBCMV plasmid
SBCWMVCPF, SBCWMVCPR, SBWMV237F
45
ABgene ABsolute QPCR ROX mastermix
540 DNAs from whole blood
PDH Taq Man
PDH human gene
PDH Probe, F, R
45
ABgene ABsolute QPCR ROX mastermix
600 NAs from whole blood 400 NAs from plasma minipools
XMRV/pMuLV RT Taq Man with BMV RT Taq Man
gag BMV
P2, F3, R4 BMV Probe, F, R
45
Qiagen QuantiTect Probe RT-PCR kit
The TaqMan assay conditions were 15 min at 95°C (15 secs 95°C, 1 min 60°C) × cycles. 400 nM concentrations of primers, and 200 nM probes were used in all the TaqMan assays with the exception of the CDC MuLV Taq Man where the concentration of each probe was 100 nM and the PDH TaqMan where the primer concentrations were 50 nM.