Inhibition of Indoleamine-2,3-dioxygenase (IDO) in Glioblastoma Cells by Oncolytic Herpes Simplex Virus
Figure 2
JD0G infection of glioblastoma cells results in efficient virus replication and tumor-selective cell death. (a) The replication of JD0G was evaluated in comparison to KOS and MGH2. HEL, U251, and SNB19 cell lines were infected in parallel with KOS, MGH2, or JD0G at an MOI of 0.1 based on U2OS titers. Supernatants were collected at 24 hpi, and the amount of virus produced was assessed by titration on U2OS cells. The data represent the average of three independent infections, and error bars represent the standard deviation. For all three viruses the differences in virus yield observed between HEL cells and either glioma cell line are statistically significant as determined by the student -test ( values less than 0.03). (b) Tumor-specific cell death was assessed by counting the number of surviving cells following infection with either JD0G or MGH2 virus. HEL, U251 or SNB19 cells were infected at an MOI of 0.1, and Trypan Blue staining was performed to count the number of viable cells 48 h postinfection. The data represent the average of three independent infections, and error bars represent the standard deviation. All differences are statistically significant ( values less than 0.01).
