Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein

Figure 5

X-Alpha galactosidase assay for interaction confirmation. All possible interacting pairs of CHPV nucleocapsid protein with other viral proteins with controls were plated in an array format on plate containing X-𝛼-gal (SD/-Trp/-Leu/-His/𝛼-gal). Interactions were considered in both directions, that is, as BD and AD fusions. The results obtained were consistent in both directions except for BD-P which was autoactivating (sectors; 14, 18, and 22). Production of blue color reconfirmed the positive interactions observed in Y2H analysis while absence of color and growth is an indication of a noninteracting protein pair.