Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein

Figure 6

Validation of N protein fragment interactions with CHPV full-length proteins by ELISA. ELISA revealing the interaction between N1, N2, and N3 and full-length CHPV N, P, M, and G proteins. Only Strep tag fusion proteins (Strep-N, Strep-P, Strep-M, Strep-G, and Strep-nsP1) and only GST tag fusion proteins (GST-N1, GST-N2, GST-N3, and GST-P) were considered as background controls. A nonrelevant Strep tag fusion protein (Strep-nsP1) of Chikungunya virus was taken to test the specificity of N1 interactions. The data shown is mean of three independent experiments (mean ± SD). The known interaction pairs (PN and PP) and noninteractors (PM and PG) of CHPV were included as experimental controls. The absorbance at 450 nm is plotted on the 𝑦 axis (10 division = 0.2 OD) and the test protein pairs are considered on the 𝑥 axis.