Elucidating the Interacting Domains of Chandipura Virus Nucleocapsid Protein
Table 1
Primers used for cloning N gene domains of CHPV.
S. no.
Construct
Oligo name
Primer sequence
1
BD-N1 and AD-N1
N1 F NdeI (BD/AD)
GGAAGTGACATATGAGTTCTCAAGTATTCTGC
N1 R BamHI (BD/AD)
GCTAACAGGATCCGAAGAATGCCCCTGGAAAC
2
BD-N2 and AD-N2
N2 F NdeI (BD/AD)
GGAAGTGACATATGGAAGACCCAGTGGAGTTTC
N2 R BamHI (BD/AD)
GCTAACAGGATCCATGGAAACTGGGATTTTTTGTTG
3
BD-N3 and AD-N3
N3 F NdeI (BD/AD)
GGAAGTGACATATGACTCTGTCACACCTCCAG
N3 R BamHI (BD/AD)
GCTAACAGGATCCTCATGCAAAGAGTTTCCTGGC
4
GST-N1
N1 F BamHI (pGEX-4T3)
GCTAACAGGATCCATGAGTTCTCAAGTATTCTGC
N1 R XhoI (pGEX-4T3)
GCTAACACTCGAGGAAGAATGCCCCTGGAAAC
5
GST-N2
N2 F BamHI (pGEX-4T3)
GCTAACAGGATCCATGGAAGACCCAGTGGAGTTTC
N2 R XhoI (pGEX-4T3)
GCTAACACTCGAGATGGAAACTGGGATTTTTTGTTG
6
GST-N3
N3 F BamHI (pGEX-4T3)
GCTAACAGGATCCATGACTCTGTCACACCTCCAG
N3 R XhoI (pGEX-4T3)
GCTAACACTCGAGTCATGCAAAGAGTTTCCTGGC
Primers used for PCR amplification of Chandipura Virus N1, N2, and N3 domains of nucleocapsid gene (F: forward primer and R: reverse primer). The names of the restriction enzymes are in italics and their recognition sequences in bold.
