Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 2

Determination of cell surface expression of escape mutant glycoproteins. Vero-SLAM cells infected with the escape mutant viruses or transfected with phCMV plasmids expressing the mutated H or mutated F proteins in the presence, when necessary, of the antifusion tripeptide FIP were lysed 1 day after infection (or after transfection) with or without previous biotinylation to extract the surface proteins or the total proteins, respectively. Western blot analysis was then done to detect the H and F proteins using anti-H mAb BH195 and anti-F mAb CB. (A) Cells infected with escape mutant viruses: (a) noninfected cells, (b) normal Halle, (c) Halle-CD46 1.2, (d) Halle-CD46 1.3, (e) Halle-CD46 3.3, (f) Halle-SLAM 1.2, (g) Halle-SLAM 2.3, and (h) Halle-SLAM 5.1. (B) Cells transfected with phCMV plasmids expressing H proteins of escape mutants: (a) normal Halle-F (control), (b) normal Halle-H, (c) Halle-CD46 1.3 H, (d) Halle-CD46 3.3 H, (e) Halle-SLAM 1.2 H, and (f) Halle-SLAM 2.3 H. (C) Western blot to detect the expression of Halle-SLAM 5.1 F; (a) normal Halle-H, (b) normal Halle-F, and (c) Halle-SLAM 5.1 F.