Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 5

Flow cytometry analysis of escape mutant Halle-CD46 1.2 (H: no change; F: M4I, V283A; M: No change). Vero-SLAM cells infected with the escape mutant virus or transfected with phCMV plasmid expressing the mutated F protein, either alone or with a phCMV plasmid expressing the nonmutated H protein, in the presence of antifusion tri-peptide FIP, were analysed 1 day afterinfection (or after transfection) using the indicated antibodies. Each overlay histogram represents one experiment; the MFI histogram data represent the mean percentages ± standard deviations for three experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle proteins as controls. (a) and (b)) Escape mutant Halle-CD46 1.2-infected cells; (c) and (d)) cells transfected with phCMV.F.M4I/V283A; ((e) and (f)) and ((g) and (h)) cells transfected with phCMV.H + phCMV.F.M4I/V283A or phCMV.F.V283A, respectively.