Research Article
Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera
Table 1
Mutations present in the H, F, and M genes of MV-G954 escape mutants obtained by in vitro immune selection.
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Escape mutants were selected using Vero-SLAM cells and their H, F, and M genes were then sequenced. The numbers 1–6 refer to the different sera from healthy MV-vaccinated donors used for selection; SSPE refers to the SSPE serum. Escape mutant mutations also found in SSPE cases are in bold. NB: for the G-954 control, two selection steps to isolate G-954 clones were made without sera. Three G-954 clones were isolated and sequencing showed no mutations in their H, F, or M genes. Strikingly, 12 of the 14 escape mutants (86%) contain at least one “SSPE mutation”; that is, a mutation that has also been found in an SSPE case. 42% of the H proteins, 57% of the M proteins, and 36% of the F proteins from the escape mutants contain at least one “SSPE mutation.” |