Research Article
Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera
Table 2
Mutations present in the H, F, and M genes of MV-Halle escape mutants obtained by in vitro immune selection.
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CD46-dependant escape mutants were selected using Vero cells and SLAM-dependant escape mutants with CHO-SLAM cells and their H, F, and M genes were then sequenced. The numbers 1–6 refer to the different sera from healthy MV-vaccinated donors used for selection; SSPE refers to the SSPE serum. Escape mutant mutations also found in SSPE cases are in bold. The “Shift” column refers to flow cytometry studies made on the mutants. The Mean Fluorescence Intensity (MFI) values of the mutants were compared with that of Halle (non-mutated control). A plus sign in the “shift” column indicates those mutants having the capacity to induce a statistically important reduction (shift) in the MFI in interaction with any of the sera or specific anti-MV antibodies. Arrows indicate the six escape mutants with a minimal number of mutations that were selected for dissection studies. NB: for the Halle control, two selection steps to isolate Halle clones were made without sera. Three Halle clones were isolated and sequencing showed no mutations in their H, F, or M genes. |