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Advances in Virology
Volume 2014 (2014), Article ID 606201, 5 pages
http://dx.doi.org/10.1155/2014/606201
Research Article

Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan

1Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan
2Center of Biotechnology & Microbiology, University of Peshawar, Peshawar 25120, Pakistan
3Department of Biotechnology, KUST, Kohat 26000, Pakistan
4Department of Botany, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan
5Department of Agriculture, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan
6Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan
7Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan

Received 14 November 2013; Revised 14 January 2014; Accepted 15 January 2014; Published 26 February 2014

Academic Editor: Jay C. Brown

Copyright © 2014 Sajid Ali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infection, including epidemiology, pathogenesis, and response to antiviral therapy. 1419 individuals were screened for anti-HCV in this study, of which 166 (11.7%) were found reactive by ICT (Immunochromatographic test). These 166 anti-HCV positive and 26 normal individuals were further analyzed. RNA was extracted from serum and reverse-transcribed to cDNA and the core region of HCV genome was targeted and amplified by multiplex PCR. HCV RNA was detected in 121 individuals, of which 87 were male and 34 were female. Genotype 3a was the most prevalent among all the genotypes observed followed by 3b. Genotypes 1a, 2a, and 2b were found in 10.89%, 13.22%, and 6.61% patients, respectively. 25.41% of the HCV RNA positive samples were not typed. 6.05% of patients were found having mixed genotypes. These findings will not only help the physicians to prescribe more appropriate treatment for the HCV infection but will also draw the attention of health-related policy makers to devise strategies to curb the disease more effectively.