Screening of Viral Pathogens from Pediatric Ileal Tissue Samples after Vaccination

Table 2

PCR assay primer sequences and expected amplicon sizes. The primers for echovirus, TTV like minivirus, small anellovirus, parechovirus, and rotavirus A were designed by LLNL as part of this study. The primers for PCV-2 were obtained from McClenahan et al. [25].

Oligo name	Sequence	Amplicon Size
Echovirus9_F	GCC CCT GAA TGC GGC TAA	112 bp
Echovirus9_R	AAA CAC GGA CAC CCA AAG TAG T
TTV-like mini_F	CGA ATG GCT GAG TTT ATG CC	146 bp
TTV-like mini_R	GTT TCT TGC CCG TTC CGC
Small anello_F	CTG AGT TTA CCC CGC TAG AC	118 bp
Small anello_R	CCG AAT TGC CCC TAG ACC
Parechovirus_F	CCC AYG AAG GAT GCC CAG	113 bp
Parechovirus_R	TTG GCC CAC TAG ACG TTT T
Rotavirus_seg10_F	CCA ADW GAA GTG ACY GCA	130 bp
Rotavirus_seg10_R	GCG ATA TGR YTG ACT DTG GCT
PCV2_F	AGCAATCAGAYCCCGTTG	84 bp
PCV2_R	CCAAGGAVGTAATCCTCCGATA