Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation

Figure 3

Decreased Akt activation does not account for the ability of NFV to inhibit HSV-1 viral replication. HSV-1 infection of Vero cells resulted in an increase in levels of phosphorylated (p)-Akt at 2 hours compared to uninfected cells. Although the Akt inhibitor LY294002 could completely suppress Akt phosphorylation in HSV-1 infected cells, NFV did not substantially reduce the levels of p-Akt even at drugs concentrations that potently block virus production.