Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle

Table 1

PCR amplification protocol.

Step	Temperature	Duration	Cycles
Initial denaturation	95°C	3 min	1
Denaturation	95°C	15 sec	35
Annealing	53.5°C	15 sec
Extension	72°C	15 sec
Final extension	72°C	1 min/kb	1