Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein
Figure 4
Displacement of from holoFbp (25 M) by 1 : 50 Nb : NTA under the same conditions as Figure 2(a). Molar ratio of Nb/Fbp (from top to bottom) is 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, and 33. insert shows the percentage of bound to the protein calculated from the LMCT band at 465 nm. Little is displaced by (open circles) whereas 1 : 50 Nb : NTA gives rise to almost complete displacement of from holoFbp (filled circles).
