Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein
Figure 5
Displacement of from Nb2-Fbp (prepared by incubating apoFbp (100 M) with 10 mol equiv 1 : 2 Nb : NTA under the same conditions as Figure 2(a)) by 1 : 2 Fe : NTA in the same buffer at 310 K. the band at ca.465 nm increases in intensity with the addition of . Molar ratios from bottom to top: are 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 4, 8, and 10. Insert shows variation in percentage of bound to the protein calculated from the LMCT band at 465 nm with the amount of added .
