Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein
Figure 6
Characterization of -FBP (apo-Fbp reloaded with [Cp2Nb(OH)Cl2] (a) or [Nb(NTA)2] (b)) by chromatography on a MonoS HR 5/5 column. The left axis shows the absorption profile at 280 nm, and the right axis is the percentage of buffer B (10 mM Hepes, 1 M NaCl, pH7.5). Green line: gradient of the buffer B applied during the elution process; cyan line: absorbance at 280 nm.
