Research Article
Zinc Binding Properties of Engineered RING Finger Domain of Arkadia E3 Ubiquitin Ligase
Table 1
Samples of each construct spiked with Zn prepared and analyzed.
| | Sample | Volume of the unknown protein sample used (l) | Final volume of the sample (ml) | Spiked Concentration of Zn (ppm) |
| | RING-H2 construct | | A | 15.0 | 5.0 | 0 | | B | 15.0 | 5.0 | 0.2 | | C | 15.0 | 5.0 | 0.4 | | D | 15.0 | 5.0 | 0.6 | | Mutant RING construct (C955R) | | A | 9.0 | 2.0 | 0 | | B | 9.0 | 2.0 | 0.1 | | C | 9.0 | 2.0 | 0.2 |
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