Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite
Figure 5
Immunolocalization of decorin (panels a and c, green) and cellular nuclei (panels b and d, blue) in the static culture (panels a and b) and in the ultrasonic culture (panels c and d), bars equal to 80 m. During the culture period, in the control (panels a and b), the osteoblasts produced a very little amount of decorin, a key regulator for matrix spatial organization, whereas, in the stimulated culture (panels c and d), the osteoblasts secreted a larger amount of 3D organized bone matrices.
