The Zn- or Cu-Thionein Character of a Metallothionein Determines Its Metal Load When Synthesized in Physiological (Metal-Unsupplemented) Conditions

Table 1

Amino acid sequences of the four MT isoforms analyzed in this work. MtnA and MtnB are two Drosophila melanogaster isoforms. Crs5 is a Saccharomyces cerevisiae MT and mMT1 is the mouse MT isoform 1. The GST-based expression system used for recombinant synthesis added two N-terminal residues to these sequences (GS), which was previously shown not to influence the binding properties of MT peptides [7], but it has to be considered for the calculation of their theoretical molecular masses (Table 2, Figure 1).

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