Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin

Figure 1

CD spectra of Cu(II) titration of HSA (0.5 mM in 0.1 M NaCl, pH 7.4), from 0 to 3 mol equivalents, at a 0.25 mol equivalent step.