Selectivity of Inhibition of N-Succinyl-l,l-Diaminopimelic Acid Desuccinylase in Bacteria: The product of dapE-gene Is Not the Target of l-Captopril Antimicrobial Activity

Table 1

Summary of strains and plasmids used in this work.

Plasmids	Genotype	Remarks
pCM655/; DapE	Plasmid with dapE gene	Allows overexpression of DapE, thus rescuing DapE knockout strains (even in absence of mDAP supplementation). Also confers ampicillin-resistance
pCM699	Plasmid without dapE gene	Confers ampicillin-resistance, but does not genetically rescue DapE knockout strains
S. enterica strains
TN5911	dapE knockout strain	Strain does not grow in absence of mDAP supplementation
TN5935	TN5911 + pCM655/DapE	DapE is overexpressed from plasmid and strain can grow in absence of mDAP supplementation
TN5959	TN5911 + pCM699	Plasmid does not contain DapE and knockout strain does not grow in absence of mDAP supplementation
E. coli strains
XL1-Blue	Wildtype (from Stratagene)	Has endogenous DapE and can grow in absence of mDAP supplementation
TN5960	XL1-Blue + pCM699	Plasmid does not contain DapE, but strain can grow in absence of mDAP supplementation due to endogenous DapE
TN5962	XL1-Blue + pCM655/DapE	In addition to endogenous DapE, this strain has plasmid-encoded DapE overexpression (from S. enterica).