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Bioinorganic Chemistry and Applications
Volume 2011 (2011), Article ID 687571, 7 pages
Research Article

Identification of Binding Mode of a Platinum (II) Complex, P t C l 𝟐 (DIP), and Calf Thymus DNA

Department of Chemistry, Faculty of Science, Razi University, Kermanshah 74155, Iran

Received 28 July 2011; Revised 17 August 2011; Accepted 23 August 2011

Academic Editor: Albrecht Messerschmidt

Copyright © 2011 Nahid Shahabadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The Pt(II) complex, PtCl2(DIP) (DIP = chelating dinitrogen ligand: 4,7-diphenyl-1,10-phenanthroline), was synthesized and characterized by elemental analysis (CHN) and 1 H NMR and UV-vis techniques. The binding of this complex to calf thymus DNA was investigated using various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA (CT-DNA): increase in melting temperature, sharp increase in specific viscosity of DNA, and induced CD spectral changes. Also the fluorescence spectral characteristics and interaction of Pt complex with DNA have been studied. Pt bound to DNA showed a marked decrease in the fluorescence intensity. The results show that both the complex and the NR molecules can intercalate competitively into the DNA double-helix structure. The experimental results show that the mode of binding of the this complex to DNA is classical intercalation.