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Bioinorganic Chemistry and Applications
Volume 2011 (2011), Article ID 898726, 5 pages
Antioxidant Activities of Plumbagin and Its Cu (II) Complex
1Department of Chemistry and Biology, Yulin Normal College, Yulin 537000, China
2The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Ministry of Education, School of Chemistry, Chemical Engineering, Guangxi Normal University, Guilin 541004, China
Received 31 July 2011; Accepted 19 August 2011
Academic Editor: Zhe-Sheng Chen
Copyright © 2011 Mingxiong Tan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Plumbagin and its Cu (II) complex [Cu (plumbagin)2]·H2O have been synthesized, and their antioxidant activities towards the inhibitory effect on DPPH free radical, reducing power, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. Plumbagin and its Cu (II) complex were found to exhibit scavenging activities on DPPH radical with the inhibitory rate of 41% and 24%, respectively. The reducing power of plumbagin was outstanding at the concentrations of 1.0, 1.5, and 2.0 mg/mL, compared to Cu (II) complex and synthetic antioxidant 2,6-di-ter-butyl-4-methylphenol (BHT); the highest level reached 1.333 for plumbagin and 0.581 for Cu (II) complex. Also, the inhibition on lipid peroxidation of plumbagin was higher than that of Cu (II) complex and BHT, 46.4% for plumbagin and 24.5% for Cu (II) complex. The results give a strong impact for designing anticancer drugs, combined with their potential cytotoxic and antioxidant activities, which can be targeted selectively against cancer cells and increase their therapeutic index and additional advantages over other anticancer drugs.
Recently, a large number of metal complexes have been designed and tested for anticancer activity and for supportive therapy in cancer patients, including the anticancer drugs doxorubicin, mitoxantrone, bleomycin, and hydroxyurea [1–3]. There is a major debate at present that natural products with diverse bioactivities are becoming an important source of potential (pro)drug chelators [4, 5]. Many naturally occurring molecules such as flavonoids, phenols, and quinones have metal chelating properties, cytotoxic, and antioxidant properties, including the inhibitory effect on free radicals and other damaging oxygen activated products such as the hydroxyl radical, superoxide, hydrogen peroxide, and lipid peroxides [6, 7]. Thus, the prospect of designing chelating prodrugs can be targeted selectively and activated against cancer cells combined with their potential cytotoxic and antioxidant activities increasing their therapeutic index and provides additional advantages over other anticancer drugs [8–10].
Our earlier studies have reported the synthesis and anticancer activities of plumbagin (Scheme 1) and its Cu (II) complex (Scheme 2). Plumbagin structurally derived from naphthoquinone was extracted from Plumbago zeylanica L, a Chinese traditional medicine. It was found that both plumbagin and its Cu (II) complex exhibited significant antitumor activities against seven human tumour cell lines (BEL-7404, NCI-H460, CNE-2, 786-O, MCF-7, HCT-116, and Hep-G2), and Cu (II) complex possessed significantly enhanced antitumor with respect to the free ligand Cu (II) ion plays an important role . All of these results make it interesting to study the antioxidant activities of plumbagin and its Cu (II) complex.
2. Materials and Methods
UV-Vis absorption spectra were performed on a Varian Cary100 UV-Visible spectrophotometer. 2,2′-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Sigma Chemicals Co. All the metallic salts were purchased from Alfa Co. Ltd. The solvents were analytical grade. All the materials were used as received without further purification unless noted specifically.
Plant material, roots of P. zeylanica, was collected in Guangxi province and identified by Prof. Tang (Institute of Life Science, Guangxi Normal University).
2.1. Isolation and Structure Identification of PLN
The roots of P. zeylanica (15 kg) were extracted with 95% EtOH. The solvent was concentrated in vacuo, and the residue was successively partitioned between H2O and n-hexane followed by EtOAc. The EtOAc extract (252 g) was subjected to silica gel cc with a gradient of EtOAc in n-hexane, eluting with n-hexane-EtOAc (9 : 1) to yield an orange crystal, plumbagin (1.33 g).
2.2. Synthesis of [Cu(Plumbagin)2]·H2O
This yellowish brown of plumbagin Cu (II) complex was synthesized by the same method as the reference described by Chen et al. .
2.3. Scavenging Activity on DPPH Radical
To evaluate the free radical scavenging activity, plumbagin or Cu (II) complex solution was allowed to react with a stable free radical, 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) . 1 mg/mL of plumbagin or Cu (II) complex in different reaction times (10, 20, 30, 40, 50, and 60 min) was assayed, and 1 mg/mL of synthetic antioxidant 2,6-di-ter-butyl-4-methylphenol (BHT) used for comparison. The reaction mixture was incubated at 25°C. The scavenging activity on DPPH radical was determined by measuring the absorbance at 515 nm each 10 min until the reaction reached the steady state. The antioxidant activity was expressed as a percentage of scavenging activity on DPPH radical: SC% = [1 − (absorbance of sample)/(absorbance of control)] × 100%.
2.4. Reducing Power
The determination of reducing power was performed as described by Oyaizu . The reducing power of various plumbagin and Cu (II) complex solutions (0.5, 1.0, 1.5, and 2.0 mg/mL) was investigated; BHT was also determined for comparison. The various samples were mixed with phosphate buffer (2.5 mL, 0.2 M, pH 6.6), potassium ferricyanide (2.5 mL, 1%) was incubated at 25°C for 20 min, trichloroacetic acid was added, and the mixture was centrifuged at 3000 rpm for 10 min. The upper layer of solution (2.5 mL) was mixed with distilled water (2.5 mL) and ferric chloride (0.5 mL, 0.1%), then the absorbance was measured at 695 nm against a blank.
2.5. Determination of Total Antioxidant Capacity
The total antioxidant capacity was determined according to the method of Prieto . Plumbagin or Cu (II) complex (0.5 mg/mL) in different reaction times (20, 40, 60, 80, 100, and 120 min) was combined with 0.3 mL of reagent solution (0.6 M sulphuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate). The reaction mixture was incubated at 95°C for 150 min. After the mixture had been cooled to room temperature, the absorbance of the mixture was measured at 595 nm against a blank. The readings were taken each 30 min. The antioxidant activity of BHT (0.5 mg/mL) was also assayed for comparison.
2.6. Antioxidant Potential of Plumbagin or Cu (II) Complex in Peanut Oil
Calculated amounts of plumbagin or Cu (II) complex solution were added to 50 mL of peanut oil. The additive was mixed into the oil with a magnetic stirrer. The oxidative deterioration of samples was studied using Schaal oven test method as described by Economou et al. . The oil samples (50 mL each) were placed in open 100 mL beakers and placed in 60 ± 0.5°C oven for 24 h. The sample (1 mg/mL) was prepared under the same conditions treated with different reaction times (1, 2, 3, 4, 5, and 6 days), BHT (1 mg/mL) was also assayed for comparison. The rate of antioxidant of peanut oil was estimated according to the increase of 2-thiobarbituric acid-reactive substances (TBARS) using the classical TBA procedure. The TBARS values of untreated and treated samples were used to calculate the inhibition of lipid oxidation as follows: Inhibition (%) = (control-treatment)/control × 100%.
2.7. Statistical Analysis
All experimental results were centred at using three parallel measurements of mean ± SD. Analysis of variance was performed by ANOVA procedure. Duncan’s new multiple-range test was used to determine the differences of means. P values < 0.05 were regarded as significant and P values < 0.01 as very significant.
3. Results and Discussion
3.1. Scavenging Activity on DPPH Radical
Antioxidant properties, especially radical scavenging activities, are very important due to the deleterious role of free radicals in biological system. DPPH is a kind of stable free radical and accepts an electron or hydrogen radical to become a stable diamagnetic molecule , which was widely used to investigate radical scavenging activity now for its advantage of ease and economy. In DPPH radical scavenging assay, antioxidants are able to reduce the stable DPPH radical to yellow-coloured, and the antioxidant power is indicated by the degree of discoloration which could be determined by measuring of a decrease in the absorbance at 515 nm.
As Figure 1 shows, at the concentration of 1 mg/mL of plumbagin or Cu (II) complex possessed scavenging activities on DPPH radical with the inhibitory rate of 41% and 24%, respectively, their scavenging effect increased with increasing reaction time, and plumbagin scavenging activities are pretty, more higher than its Cu (II) complex and BHT, indicating that the presence of Cu (II) decreased the scavenging effect of DPPH radical.
3.2. Reducing Power
The reducing capacity of a compound may serve as a significant indicator of its potential antioxidant activity . In the assay, the presence of reductants in the antioxidant sample causes the reduction of the Fe3+/ferricyanide complex to the Fe2+/ferrous form , so the reducing power of the sample could be monitored by measuring the formation of Perl’s Prussian blue at 695 nm . As Figure 2 shows, the reducing properties of plumbagin and Cu (II) complex at different concentration exhibited correlation with increasing concentration. The reducing power of plumbagin was outstanding at the concentration of 1.5 and 2.0 mg/mL compared to Cu (II) complex and BHT. At the concentration of 2.0 mg/mL, the reducing power of plumbagin and Cu (II) complex reached 1.333 and 0.581, respectively. It was speculated that the decreased antioxidant power of Cu (II) complex, compared to plumbagin, may be the absence of phenolic group due to forming the complex, thus the presence of the active hydrogen in phenolic hydroxyl group was likely to contribute considerably to the observed antioxidant effects.
3.3. Total Antioxidant Capacity Assay
The assay is based on the reduction of Mo (VI) to Mo (V) by plumbagin and Cu (II) complex in different reaction times (20, 40, 60, 80, 100, and 120 min) and subsequent formation of a green phosphate/Mo (V) complex at acid pH. The high absorbance values indicated that the sample possessed significant antioxidant activity. As Figure 3 shows, plumbagin and Cu (II) had significant total antioxidant activities compared to BHT, and the effects increased with increasing reaction time, while total antioxidant capacity of Cu (II) complex was superior to plumbagin.
3.4. Lipid Peroxidation in Peanut Oil
The level of 2-thiobarbituric acid-reactive substances (TBARS), products of lipid peroxidation, is often measured in order to assess the extent of oxidation that occurs in biological systems. Peanut oil was treated with plumbagin, Cu (II), and BHT complex at the concentration of 1 mg/mL in different reaction times (1, 2, 3, 4, 5, and 6 days). As shown in Figure 4, the inhibition rate of lipid oxidation between plumbagin and Cu (II) complex had significant effects and was enhanced with increasing reaction time, the highest level reached 46.4% for plumbagin and 24.5% for Cu (II) complex after 6 days, and the lipid oxidation inhibition of Cu (II) complex is lower than that of PLN and BHT at the same period.
In this study, we have investigated the antioxidant possibility of plumbagin and its Cu (II) complex. The results indicated that both the compounds exhibited some antioxidant activities, including the inhibitory effect on DPPH free radical, reducing power, total antioxidant capacity, and lipid peroxidation, which plumbagin possessed higher abilities than that of Cu (II) complex and BHT at the same condition towards DPPH free radical, reducing power, and lipid peroxidation. It was speculated that the decreased antioxidant power of Cu (II) complex compared to plumbagin may be the absence of phenolic group due to forming the complex, thus the presence of the active hydrogen in phenolic hydroxyl group was likely to contribute considerably to the observed antioxidant effects. Also, this study gives a strong impact for designing anticancer drugs, combined with their potential cytotoxic and antioxidant activities, which can be targeted selectively against cancer cells and increase their therapeutic index and additional advantages over other anticancer drugs.
This work was supported by the National Natural Science Foundation of China (no. 81060360), the Natural Science Foundation of Guangxi Province (no. 2010-013064 and 2009-0991284), the Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China (no. CMEMR2011-09), and the Scientific Researches Foundation of Educational Committee of Guangxi Province (no. 200808LX407).
- J. Blatt, S. R. Taylor, and G. J. Kontoghiorghes, “Comparison of activity of deferoxamine with that of oral iron chelators against human neuroblastoma cell lines,” Cancer Research, vol. 49, no. 11, pp. 2925–2927, 1989.
- G. J. Kontoghiorghes, K. Pattichis, K. Neocleous, and A. Kolnagou, “The design and development of deferiprone (L1) and other iron chelators for clinical use: targeting methods and application prospects,” Current Medicinal Chemistry, vol. 11, no. 16, pp. 2161–2183, 2004.
- M. F. Primik, G. Mühlgassner, M. A. Jakupec et al., “Highly cytotoxic copper(II) complexes with modified paullone ligands,” Inorganic Chemistry, vol. 49, no. 1, pp. 302–311, 2010.
- L. Wang, S. Gou, Y. Chen, and Y. Liu, “Potential new antitumor agents from an innovative combination of camphorato, a ramification of traditional Chinese medicine, with a platinum moiety,” Bioorganic and Medicinal Chemistry Letters, vol. 15, no. 14, pp. 3417–3422, 2005.
- L. Kuo-Hsiung, “Discovery and development of natural product-derived chemotherapeutic agents based on a medicinal chemistry approach,” Journal of Natural Products, vol. 73, no. 3, pp. 500–516, 2010.
- K. Slinkard and V. L. Singleton, “Total phenol analyses: automation and comparison with manual methods,” American Journal of Enology and Viticulture, vol. 28, no. 1, pp. 49–55, 1977.
- M. X. Tan, J. C. Zhu, Y. M. Pan, and H. S. Wang, “Synthesis, cytotoxic activity, and DNA binding properties of copper (II) complexes with hesperetin, naringenin, and apigenin,” Bioinorganic Chemistry and Applications, vol. 2009, Article ID 347872, 2009.
- G. K. Kontoghiorghes, M. I. Jackson, and J. Lunec, “In vitro screening of iron chelators using models of free radical damage,” Free Radical Research Communications, vol. 2, no. 1-2, pp. 115–124, 1986.
- S. Kummar, M. Gutierrez, and J. H. Doroshow, “Drug development in oncology: classical cytotoxics and molecularly targeted agents,” British Journal of Clinical Pharmacology, vol. 62, no. 1, pp. 15–26, 2006.
- S. Blois, “Antioxidant determinations by the use of a stable free radical,” Nature, vol. 181, no. 4617, pp. 1199–1200, 1958.
- Z. F. Chen, M. X. Tan, L. M. Liu, H. Liang, and O. Chris, “Cytotoxicity of the traditional chinese medicine (TCM) plumbagin in its copper chemistry,” Dalton Transactions, vol. 28, no. 48, pp. 10824–10833, 2009.
- C. Sanchez-Moreno, J. A. Larrauri, and F. Saura-Calixto, “A procedure to measure the antiradical efficiency of polyphenols,” Journal of the Science of Food and Agriculture, vol. 76, no. 2, pp. 270–276, 1998.
- M. Oyaizu, “Studies on product of browning reaction prepared from glucose amine,” Japanese Journal of Nutrition, vol. 44, pp. 307–315, 1986.
- P. Prieto, M. Pineda, and M. Aguilar, “Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E,” Analytical Biochemistry, vol. 269, no. 2, pp. 337–341, 1999.
- K. D. Economou, V. Oreopoulou, and C. D. Thomopoulos, “Antioxidant activity of some plant extracts of the family labiatae,” Journal of the American Oil Chemists Society, vol. 68, no. 2, pp. 109–113, 1991.
- A. Yildirim, A. Mavi, M. Oktay, A. A. Kara, F. Algur, and V. Bilaloglu, “Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts,” Journal of Agricultural and Food Chemistry, vol. 48, no. 10, pp. 5030–5034, 2000.
- I. Gülcin, “Antioxidant and antiradical activities of L-carnitine,” Life Sciences, vol. 78, no. 8, pp. 803–811, 2006.
- Y. C. Chung, C. T. Chang, W. W. Chao, C. F. Lin, and S. T. Chou, “Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1,” Journal of Agricultural and Food Chemistry, vol. 50, no. 8, pp. 2454–2458, 2002.