Research Article

DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7

Figure 3

(a) Representative image of agarose gel electrophoresis of the cleavage assay. Only HCPT induced the formation of a slow migrating complex formed by enzyme, drug, and DNA, whereas Van-7 did not. (b) Comet assay performed in isolated nuclei in the presence of the vehicle, HCPT (40 μM), or Van-7 (40 μM). Tail moment for each comet was calculated. Nuclei were grouped according to the tail moment value in three groups, and the frequency distribution is shown in the bar chart. DNA damage is increased in nuclei with the highest tail moment values.
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756374.fig.003b
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