Norfloxacin and N-Donor Mixed-Ligand Copper(II) Complexes: Synthesis, Albumin Interaction, and Anti-Trypanosoma cruzi Activity

Table 2

Activity (mean ± SD) and selectivity index (SI) of the compounds and benznidazole upon bloodstream trypomastigotes (BT) forms of T. cruzi (Y strain) in vitro (24 h of incubation at 37°C).

Compound	EC50 (μM)	SI
Norfloxacin (NOR)	126 ± 30	>1.25
CuCl(bipy)(NOR)Cl·2H2O (1)	16 ± 4	4
CuCl2(phen)(NOR)·3H2O (2)	4.4 ± 1.4	2.68
CuCl2(bipy)	14 ± 7	4.5
CuCl2(phen)	7 ± 5	<4
CuCl2(NOR)	78 ± 12	0.8
	83 ± 3	6
Benznidazole	13 ± 2	77

SD: standard deviation of multiple experimental measurements.
SI: selective index: ratio between LC₅₀/EC₅₀ values.
LC₅₀: drug concentration which reduces the viability of mammalian cell by 50%.
EC₅₀: drug concentration which reduces the number of the parasites by 50%.
Data published in Martins et al., 2012 [14].