Co(II) Coordination in Prokaryotic Zinc Finger Domains as Revealed by UV-Vis Spectroscopy

Figure 2

(Top) Ros87_C27D amino acid sequence; (a) UV-Vis spectra of Ros87 titration with CoCl₂; (b) the ¹H-¹⁵N HSQC spectrum of Ros87 in the presence of 1.4 equivalents of Co(II); (c) the experimental CD spectrum of Ros87_C27D (red) overlaid to the fitted CD data (blue) by the server BeStSel; the green histogram indicates the deviations; (d) secondary structure content calculated from the CD data by the server BeStSel.