Ir-6: A Novel Iridium (III) Organometallic Derivative for Inhibition of Human Platelet Activation

Figure 4

Effects of Ir-6 on PLCγ2, PKC, and Akt activation in human platelets. Washed platelets were preincubated with the solvent control (0.1% DMSO), LY294002 (10 µM), or Ir-6 (10 or 20 µM). Subsequently, either collagen (1 g/mL) or PDBu (150 nM) was added to trigger (a) platelet aggregation, (b) PLCγ2 phosphorylation, and (c) PKC activation (pleckstrin phosphorylation), or (d) Akt activation. Platelets were collected, and their subcellular extracts were analyzed to determine the levels of phosphorylation of (b) PLCγ2, (c) PKC, and (d) Akt. Data are presented as the mean ± standard error of the mean (). and , compared with the resting control; ^# and ^###, compared with the DMSO-treated group. The profiles in (a) and (d, left panel) are representative of four independent experiments.

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