Structural Basis for pH-Dependent Oligomerization of Dihydropyrimidinase from Pseudomonas aeruginosa PAO1

Figure 4

Gel filtration chromatographic analysis. Gel filtration chromatography was carried out by the AKTA-FPLC system in Buffer C (20 mM MES and 100 mM NaCl, pH 5.9). The corresponding peaks show the eluting P. aeruginosa dihydropyrimidinase. The column was calibrated with proteins of known molecular weight: thyroglobulin (670 kDa), γ-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin B₁₂ (1.35 kDa).