Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119

<div>The excitation (solid) and emission (dash) fluorescence of the Amplex Red oxidation product (resorufin) catalyzed by WT CYP119. Reaction components were 10 <i>μ</i>M Amplex Red, 1.5 mM H<sub>2</sub>O<sub>2</sub>, and 1.5 <i>μ</i>M WT CYP119 in 50 mM sodium phosphate at room temperature at pH 7.4, spectra taken after 40 minutes.</div>

Bioinorganic Chemistry and Applications

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Figure 4

Figure 4: Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119 