Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119

<div>Determination of the kinetic parameters of Amplex Red oxidation by H<sub>2</sub>O<sub>2</sub> in the presence of N-His-CYP119. Reactions contained 10 <i>μ</i>M Amplex Red, 1.5 <i>μ</i>M N-His-CYP119, and 0.2–2 mM of H<sub>2</sub>O<sub>2</sub> in 50 mM sodium phosphate buffer, pH 7.4. Initial rate is obtained from resorufin formation at 30 min.</div>

Bioinorganic Chemistry and Applications

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Figure 7

Figure 7: Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119 