Biochemistry Research International
Volume 2010 (2010), Article ID 489892, 4 pages
doi:10.1155/2010/489892
Research Article

Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus

Heather J. Montgomery, Andrea L. Dupont, Hilary E. Leivo, and J. Guy Guillemette

Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada

Received 19 July 2009; Accepted 2 September 2009

Academic Editor: Francesca Cutruzzolà

Copyright © 2010 Heather J. Montgomery et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material includes sequence alignments of bacterial NOS-like proteins and three mammalian NOS oxygenase domains. The results of our complete characterization of the the Bacillus cereus NOS-like protein are also included in the supplementary section.

  1. Supplementary Material