Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NOD/SCID versus NOD/SCID/γ chainnull Mice
Figure 3
Comparison of in vivo selected MGMTP140K-transduced human cells in NSG and NOD/SCID mice. The starting cell number was 4 × 105 cells per transplanted mouse. MGMTP140K-transduced CD34+ cells were transplanted into NS2 and NOD/SCID mice and selected in vivo as outlined in Figure 2. The BM was harvested and analyzed for the level of total human cell engraftment (%huCD45+ cells) and the level of transduced human cells (%huCD45+EGFP+) by flow cytometry. The BM cellularity was similar between the NSG and NOD/SCID mice and the differences in total human engraftment in NSG versus NOD/SCID mice were not significant (). The data presented here are from 1 transplant experiment ( mice in vehicle-treated cohorts and mice in 6BG/BCNU-treated cohorts). Similar results were obtained in a second independent transplant experiment.
