Breast Cancer Stem Cells Survive Periods of Farnesyl-Transferase Inhibitor-Induced Dormancy by Undergoing Autophagy
Figure 2
Expression of autophagy markers in FTI-treated cells. (a) Subconfluent MCF-7 cells were treated with 25 M FTI or vehicle control for 24 h, cell lysates harvested and western blot analysis performed with antibodies for autophagy-associated proteins. (b) MCF-7 cells were treated with FTI for 48 h and then 5 mM 3-MA or vehicle control added to the cultures. Cells were fixed 24 h later and a TUNEL assay performed. The arrow represents a single positive apoptotic cell.
