524845.fig.002a
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524845.fig.002b
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524845.fig.002c
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524845.fig.002d
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524845.fig.002e
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Figure 2: SP analysis of RPMI-8226 cells maintained in culture conditions for the indicated days. (a) Cell growth curve; (b) viable cell proportion determined by PI staining by flow cytometry; (c) SP proportion over the culture day; (d) SP% change with time after subculture; (e) SP flow cytometry profiles changed over the culture day. Data are mean ± SD value of triplicate samples in an independent experiment. Bar indicates standard deviation. One of the three independent experiments is shown as representative data.