Figure 5: Effect of staining conditions on viable cell and SP%. (a) Live cell proportion remained relatively stable when staining cell density increased. Cell density ranged from to cells/mL. (b) SP percentage increased with increasing cell density. (c) Live cell proportion remained stable when staining volume increased from 100 to 2000 μL. (d) SP percentage decreased with increasing staining volume. (e) The viable cell proportion prior to flow cytometry analysis was stable when increasing staining duration from 30 min to 150 min. (f) SP percentage decreased with increasing staining duration and plateaued after 90 min. (g) The viable cell proportion prior to flow cytometry analysis was stable when mixing interval time changed from 15 to 90 min. (h) SP percentage increased with the decrease of mixing interval. Data are mean ± SD value of triplicate samples in an independent experiment. Bar indicates standard deviation. The experiment was repeated at least three times with similar results. One of the three independent experiments is shown as representative data.