Inhibition of Mammalian Target of Rapamycin in Human Acute Myeloid Leukemia Cells Has Diverse Effects That Depend on the Environmental In Vitro Stress
Figure 2
Unsupervised hierarchical cluster analysis of the intracellular levels of (i) the five autophagy-involved mediators LC3B, Beclin-1, ATG3, ATG7 and ATG10, (ii) the apoptosis regulators bcl-2, bcl-XL, and bax, and (iii) the phosphorylated form of the mTOR downstream target S6RP. The levels were determined by flow cytometry for primary human AML cells derived from 9 unselected patients. Cells were incubated in either FBS-containing or serum-free medium for 4 and 24 hours before analysis. The figure presents the results for cells incubated in FBS-containing medium for 4 (a) and 24 hours (b) and for cells incubated in serum-free medium for 24 hours (c).
