Inhibition of Mammalian Target of Rapamycin in Human Acute Myeloid Leukemia Cells Has Diverse Effects That Depend on the Environmental In Vitro Stress

Figure 5

The effect of rapamycin and BMS-345541 on viability (A), lysosomal acidity (B), and cell cycle distribution (C) for the AML cell line KG1a. As is indicated at the top of the figure the AML cells were cultured for 24 hours in FBS-containing medium alone and medium supplemented with rapamycin 100 nM alone or rapamycin plus BMS-345541. The figure presents the results from flow-cytometric analyses of viability ((A) percent of viable cells is given in the diagrams), lysosomal acidity ((B) the MFI values are given in the diagrams), and cell cycle distribution ((C) percent of cells in G0/G1 phase is given in the diagrams).