Research Article

Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis

Figure 2

Von Kossa staining of cells in culture at 2 and 3 weeks. Black areas reflect the presence of mineral in the cultures. No mineralization is seen in the basic medium at 2 weeks (a) and 3 weeks (b). The noninfected rMSCs at 2 weeks (c) and 3 weeks (d) show minimal mineralization. Images of the Ad-VEGF+BMP-6 infected mixed marrow cells at 2 weeks (e) and at 3 weeks (f) showed considerable mineralization.
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(a) Basic Media—2 weeks
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(b) Basic Media—3 weeks
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(c) Osteogenic—2 weeks
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(d) Osteogenic—3 weeks
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(e) Adenovirus—2 weeks
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(f) Adenovirus—3 weeks