Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient

Figure 3

Stem cell populations were diminished significantly after spontaneous migration. Multicolor flow cytometric analyses were performed on BM-TNC after isolation (“fresh”), after cultivation (“incubation control”), or on BM-TNC retrieved from lower Boyden chamber after cultivation for 24 h (“spontaneous migration”). Cell populations (a) and stem cell subpopulations (b) were evaluated as shown in Figure 1. CD34+, CD133+, and CD117+ cells formed significantly lower percentages of BM-TNC which had undergone spontaneous migration (a). The effect was even more pronounced for populations expressing multiple stem cell markers (b). Incubation had no influence on CD34+, CD133+, and CD117+ cells or on their subpopulations but led to an increase in CD184+ cells, .

(a)

(b)