A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

Figure 2

Immunocytochemistry of cell surface markers for human bone marrow-derived MSCs. Positive markers (CD44, CD90, CD146, and STRO-1) and negative markers (CD19 and CD14) are shown as labeled in each panel. CD44 is stained green with Alexa Fluor 488 conjugated secondary antibody, whereas the secondary antibody for the rest of the panels was conjugated with Cy3 and that showed red color in positive markers or absence of red colored labeling in negative markers. Nuclei stained blue with DAPI in all the panels.