A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

Figure 5

Chondrogenic differentiation of bone marrow-derived MSCs, and generation of micromass cartilages (a–c). A high density of MSCs at 2 h shows MSCs condensation at the beginning of chondrogenesis (a). A micromass cartilage, shown in a 24-well plate, at week 4 of chondrogenesis (b). Micromass cartilage, at weeks 1, 2, 3, and 4, shows a gradual increase in size with time (c).

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