BET Inhibition Suppresses S100A8 and S100A9 Expression in Acute Myeloid Leukemia Cells and Synergises with Daunorubicin in Causing Cell Death

Figure 6

Effects of JQ1 and daunorubicin on S100A8 and S100A9 expression in OCI-AML3 cells. Cells were incubated in medium containing various concentrations of daunorubicin with or without 0.5μM JQ1 for 48 hr at 37°C. After incubation cell viability (a) was measured using WST-1 reagent and detection at 450nm or activated caspase 3 was detected by immunolabelling and caspase 3 positive cells counted (mean ± SEM, n=3). In (a) the legend corresponds to the data lines from top to bottom (b). Western blotting of (c) OCI-AML3 cells treated with lane 1: daunorubicin 0.5μM; 2: daunorubicin 2.5μM; 3: JQ1 0.5μM; 4: daunorubicin 0.5μM plus JQ1 0.5μM; 5: control for 48hr, and (d) time course of induction of S100A8 and S100A9 protein expression by 0.5μM daunorubicin. (e) shows changes in mRNA expression after 24 hr treatment with 0.5μM daunorubicin.

(a)

(b)

(c)

(d)

(e)