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Journal of Biomedicine and Biotechnology
Volume 2003 (2003), Issue 5, Pages 257-266
http://dx.doi.org/10.1155/S1110724303209220
Review article

Protein and Chemical Microarrays—Powerful Tools for Proteomics

Division of Hematology and Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California, Davis, 4501 X Street, Sacramento, CA 95817, USA

Received 11 September 2002; Accepted 10 December 2002

Copyright © 2003 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the last few years, protein and chemical microarrays have emerged as two important tools in the field of proteomics. Specific proteins, antibodies, small molecule compounds, peptides, and carbohydrates can now be immobilized on solid surfaces to form high-density microarrays. Depending on their chemical nature, immobilization of these molecules on solid support is accomplished by in situ synthesis, nonspecific adsorption, specific binding, nonspecific chemical ligation, or chemoselective ligation. These arrays of molecules can then be probed with complex analytes such as serum, total cell extracts, and whole blood. Interactions between the analytes and the immobilized array of molecules are evaluated with a number of different detection systems. In this paper, various components, methods, and applications of the protein and chemical microarray systems are reviewed.